The cysteine proteome
نویسندگان
چکیده
منابع مشابه
Homogenous Phase Enrichment of Cysteine-Containing Peptides for Improved Proteome Coverage.
We describe a proteomic reactor-based homogeneous phase enrichment of cysteine-containing peptides in a filter aided sample preparation (FASP) format. In this approach thiol-reduced proteins are derivatized with thiol-activated polyethylene glycol (TAPEG) before protein cleavage. Consecutive digestion with endoproteinase LysC and trypsin allows isolation of two fractions of nonderivatized pepti...
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ژورنال
عنوان ژورنال: Free Radical Biology and Medicine
سال: 2015
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2015.03.022